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Advances in Animal and Veterinary Sciences

Research Article
Adv. Anim. Vet. Sci. 1 (4S): 24 - 29. Special Issue-4 (Progress in Research on Viruses and Viral Diseases)
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Abidali Ismailbhai Dadawala1, Hemendra Singh Kher1, Bharat Singh Chandel1, Abidali Gulamhaider Bhagat1, Harshad Chaturbhai Chauhan1, Koushlesh Ranjan2, Prasad Minakshi2*
1College of Veterinary Science and AH, Sardarkrushinagar Dantiwada Agricultural University Sardarkrushinagar, Gujarat, India, 385506; 2Department of Animal Biotechnology, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar, Haryana, India, 125004
*Corresponding author: minakshi.abt@gmail.com

ABSTRACT
Bluetongue (BT), transmitted by Culicoides (biting midges), is a disease of domestic and wild ruminants, infecting primarily sheep. The causative agent is bluetongue virus (BTV) which is double stranded RNA virus of the genus Orbivirus and family Reoviridae. A total of 38 blood samples from goat were screened for BTV using s–ELISA. Of the samples analyzed, only 12 were found positive. The Sandwich–enzyme linked immunosorbent assay (s–ELISA) positive samples were subjected to virus isolation in chicken embryo and in BHK–21 cell culture and only 5 samples were adapted to cell culture. The viral nucleic acid was extracted and BTV specific ten segmented genome with characteristics migration pattern of 3:3:3:1 was observed via RNA–PAGE (Polyacrylamide gel electrophoresis). BTV was further confirmed with group specific NS1 gene and VP2 gene based serotyping. All the five isolates were typed as BTV16. Phylogenetic analysis revealed that the isolates in this study clustered closely with BTV16 Indian isolates and were related to BTV16 isolates from Japan and Greece. The analysis revealed nucleotide (98.5%) and deduced amino acid sequence identity (97.5%) among themselves and with other BTV16 isolates reported previously from India. They also showed nucleotide (96.5 to 97.6%) and amino acid identity (96 to 98%) with BTV16 from Japan and Greece. The least identity of nucleotide (<93.1%) and amino acid (<98%) was observed with BTV16 isolate (DPP965) from Australia.

Key Words: Bluetongue virus 16, Vp2 gene, Serotype, RT–PCR