Advances in Animal and Veterinary Sciences

Research Article
Adv. Anim. Vet. Sci. 7(s2): 183-190
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Sawsan M.A. El-Sheikh1, Abd El-Alim F. Abd El-Alim1, Hosny Abd El-Fadil Ibrahim1, Elham A. Mobarez2, Dalia M.A. El-Masry2, Walaa A. El-Sayed3

1Pharmacology Department, Faculty of Veterinary Medicine, Zagazig University, 44511, Egypt; 2Animal Health Research Institute, Dokki, Egypt; 3Animal Health Research Institute, Zagazig branch, 44516, Egypt.

Abstract | Bacterial resistance and antibiotics residues due to over and misuse of antibiotics need to overcome through formulation of novel natural-based nano antimicrobial agents. Propolis is a natural antimicrobial which is limited by its insolubility and low absorption. Chitosan nanoparticles consider promising bioactive polymers in nanomedicine regarding to their antibacterial activity, non- toxic and non- immunogenic properties which also make them ideal drug delivery agents. The present study describes the synthesis of chitosan nanoparticles and chitosan-propolis nanocomposite by a facile chemical method. The physicochemical properties of chitosan nanoparticle and nanocomposite have been characterized by FTIR fingerprint spectroscopy, zeta potential and TEM microscopies. With an assessment to the application of chitosan nanoparticles and nanocomposite on Vero cell line, the safety of these particles was determined. Characterization of chitosan nanoparticle and chitosan-propolis nanocomposite indicated the spherical phase with size 26.13 nm and 30.45 nm, respectively; for the FT-IR spectroscopic analysis; nanocomposite showed the interaction between chitosan chains molecule and propolis when compared with chitosan only, indicating the actual hydrogen bonding in many sites. The zeta potential of chitosan nanoparticle and chitosan-propolis nanocomposite was 51.0± 5.92 mV and 41.0±7.55 mV, respectively. The results decisively improved the antibacterial activity of chitosan-propolis nanocomposite from minimum inhibitory concentration (0.5 μg/ml against S. aureus, 2 μg/ml against E-coli and 4 μg/ml against Salmonella).

Keywords | Chitosan, Propolis, nanocomposite, Vero cell line, Antibacterial