Advances in Animal and Veterinary Sciences

Research Article
Adv. Anim. Vet. Sci. 7(s2): 145-153
Figure 1

(A.) Br-MSCs isolation 3rd day of culture showed rounded and elongated nucleus (B.) 7th day of culture cells showed typical fibroblastic appearance. Br-MSCs flow cytometrical identification (C.: F.) (C.) side scatter and forward scatter gated Br-MSCs cells, (D.) gated Br-MSCS cell population were positive for CD105, (E.) gated Br-MSCS cell population were positive for CD90 (F.) gated Br-MSCS cell population were negative for hematopoietic stem cell surface marker (CD34, CD45).  

Figure 2

Sperm morphology (A.: I.), A. normal sperm, B. looped tail sperm, C. detached tail sperm, D. stunt sperm, E. detached head, F. and G. bent tail H. curved tail sperm, I. broken head. 

Figure 3

Effect of Br-MSCs administration on blood glucose level, serum insulin level and testicular immune profile of type 1 induced diabetic rat (A-H). A. fasting blood glucose level, B. serum insulin level and RT-PCR analysis for relative mRNA expression of; C. IL1β, D. IL6, E. IL8, F. TGFβ1, G. PCNA and H. IL10 gene in testicular tissue. Means bearing different superscripts were significantly different at P < 0.05. Abbreviations; IL (interleukin), TGFβ1 (Transforming growth factor beta 1), PCNA (proliferating cell nuclear antigen) Gapdh (Glyceraldehyde 3-phosphate dehydrogenase).

Figure 4

effect of Br-MSCs administration on testis morphometry of type 1 induced diabetic rats (A – C): A. Photomicrograph of testes showing normal testicular tissue with preserved seminiferous tubules (black arrow) and leydig cells (yellow arrow) HandE X 100, B. Photomicrograph of testes showing, sever tubular atrophy (black arrow), edema (red star) and partially atrophied tubules (yellow arrows) HandE X 100, C. Photomicrograph of diabetic Br-MSCs treated rats testes showing mild to moderate interstitial edema (red star) with normal tubules appears normal with functional spermatogenesis, spermiogenesis (yellow arrows) and hypertrophied leydig cell (red arrow), HandE X 100.