Advances in Animal and Veterinary Sciences

Research Article
Adv. Anim. Vet. Sci. 7(s2): 117-122
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Ali A. Abdel-Rhman1*, Farouk A. El Balkemy2, Nasser Z. Abouzeid2, Samir M. Edries3

1Veterinarian in Prison Sector Ministry of Interior; 2Animal Medicine Department, Infectious Diseases, Faculty of Veterinary Medicine, Zagazig University, 44511, Egypt; 3Veterinary Serum and Vaccine Research Institute, Abassia, Cairo.

Abstract | Regular updating of our knowledge regarding canine parvovirus infection (CPV) diagnosis is crucial to increase the awareness of disease significance and subsequently to improve vaccination programs. This work aimed for isolation, identification, molecular characterization and sequencing of the CPV strain of naturally infected dogs in Egypt. A total of 120 dogs of different ages and breeds suffering from vomiting, bloody diarrhea, dehydration, and ruffled hair were clinically examined. The Rapid Immune Chromatographic Test (IC) and culturing on Vero cell were used to test fecal samples (n=120). The findings revealed that (69 and 48/ 120) samples (57.5 and 40 %) were positive for CPV using IC test and CPV’s specific cytopathic effect on Vero cell respectively. The virus identified by Rapid Slide Agglutination (RAT), Virus Neutralization Test (VNT) and Direct Fluorescent Antibody Technique (FAT). Molecular characterization was performed by using the Hfor/Hrev primers for 3 positive samples by IC alone and 3 positive samples by both IC and cell culturing in addition to 6 negative samples. The positive samples were detected at 630 bp for CPV and 427 bp for CPV-2a. Three molecularly positive samples were genotyped using specific primers for CPV-2a, CPV-2b, and CPV-2c. Current study findings revealed that the three sequenced samples are strongly close to the CPV type 2a. We could conclude that CPV-2a is the current type of CPV circulating in Egyptian dog’s population.

Keywords | Canine parvovirus, Egypt, Enteritis, Molecular characterization, Sequencing