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Advances in Animal and Veterinary Sciences

Research Article
Adv. Anim. Vet. Sci. 8(1): 11-17
 
Figure 1

PSA lectin reactivities in the low-dose secretome-treated group; reactivity was not present 1 week after the first injection, but the intensity was increased 1 week after the second and third injections. A: low-dose secretome-treated group 1 week after the first injection, B: one week after the second injection, C: one week after the third injection, arrow: lectin reactivities. 

Figure 2

PSA lectin reactivities in the control group and high-dose secretome-treated group; the reaction intensity of spermatids and spermatozoa increased every week, and the strongest intensity were shown 1 week after the fourth injection. A and B: control group, C: high-dose secretome-treated group 1 week after the first injection, D: one week after the second injection, E: one week after the third injection, F: one week after the fourth injection, arrow: lectin reactivities. 

Figure 3

LCA lectin reactivities in the control group and secretome-treated group; no reactivity was evident in spermatocytes in the secretome-treated group 1 week after the first injection (B), but at 1 week after the second injection (C), moderate reactivity was shown. In the secretome-treated group, 1 week after the fourth injection (D) very strong reactivities were evident in spermatocytes and spermatozoa. A: control group, B: low-dose secretome-treated group 1 week after the first injection, C: low-dose secretome-treated group 1 week after the second injection, D: high-dose secretome-treated group 1 week after the fourth injection, arrow: lectin reactivities. 

Figure 4

PHA-L lectin reactivities in the control and secretome-treated groups. Reactivity of PHA-L lectin was not shown in the low-dose group 1 week after the first injection (B) but was shown 1 week after the second injection (C); strong reactivities were shown in spermatids in the high-dose secretome-treated group 1 week after the fourth injection. A: control group, B: low-dose secretome-treated group 1 week after the first injection, C: low-dose secretome-treated group 1 week after the second injection, D: high-dose secretome-treated group 1 week after the fourth injection, arrow: lectin reactivities. 

Figure 5

SJA lectin reactivities in the control group and secretome-treated group; reactivity of SJA lectin was not shown in the low-dose group 1 week after the first injection (B). In the high-dose secretome-treated group 1 week after the second and third injection, the reactivities shown were moderate to strong. A: control group, B: low-dose secretome-treated group 1 week after the first injection, C: high-dose secretome-treated group 1 week after the second injection, D: high-dose secretome-treated group 1 week after the third injection, arrow: lectin reactivities.