Gel electrophoresis image showing; PCR detection of B. caballi and T. equi with a pair of universal screening primers (Bec-UF1 and Bec-UR): Lanes M) 100 bp ladder DNA marker; Lane 1-2) B. caballi in approximately band size 876bp; lane 3-6) T. equi in approximately band size 930bp; Lane N) negative control. 

Gel electrophoresis image showing: lane M) 100bp DNA ladder; Lane P) positive control for T. equi and B. caballi; Lane1-4) multiplex PCR detected T. equi and B. caballi in approximately band size 392 and 540 bp respectively; Lane N) negative control. 

Rooted phylogenic tree of Theileria equi as interfered from partial sequences of 18S rRNA gene. Black diamond indicated sequences obtained from the present study; others represent sequences from Genbank. 

Rooted phylogenic tree of Babesia caballi as interfered from partial sequences of 18S rRNA gene. Black diamond indicated sequences obtained from the present study; others represent sequences from Genbank.