Advances in Animal and Veterinary Sciences

Research Article
Adv. Anim. Vet. Sci. 7(s2): 57-62
Figure 1

IBV isolated in ECEs. Normal embryo development (a) and curled, stunted, and dwarfed embryos infected with IBV after fourth passage (b) 

Figure 2

RT-PCR amplification of partial S1 gene from IBV isolates in the present study showed a single specific band (400bp). Lane 1: 1 kbp DNA ladder (Fermantas); Lane 2, positive control; Lanes 3–6: positive IBV-infected samples. Lane 7: negative control. 

Figure 3

Comparison between deduced amino acid sequences of HVR1 (amino acid position from 60-88) and HVR2 (amino acid position from 115-140) of S1 protein of two isolates reported in the study and currently used vaccine strains in Egypt.  

Figure 4

Phylogenetic tree based on nucleotide sequences of S1 gene for the two IBV isolates and related 25 reference IBV strains. The IBV-Ch-KafrAshaykh-2017 isolate marked with a red triangle belonging to isolates of GI-23 lineage (Egy/variant1 group), while IBV Ch-Sharkia-2018 marked with a green triangle belonging to isolates of GI-1lineage (classical group).