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Advances in Animal and Veterinary Sciences

Research Article
Adv. Anim. Vet. Sci. 7(9): 791-797
Http://dx.doi.org/10.17582/journal.aavs/2019/7.9.791.797
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Akhmad Rizaldi1, Denny Widaya Lukman2*, Herwin Pisestyani2

¹Graduate School, Study Programs of Veterinary Public Health, IPB University; ²Department of Animal Infectious Diseases and Veterinary Public Health, Faculty of Veterinary Medicine, IPB University, West Java, Indonesia 16680.

Abstract | This study aimed to determine the occurrence of antibiotic resistance of Escherichia coli (E. coli) isolated from pork sold inTamiang Layang Market, East Barito District. We took a total of 41 upper thigh pork samples were taken from all of the Tamiang Layang Market pork traders. Isolation and identification of E. coli were carried out using Brilliance E. coli/Coliform selective Agar, Eosin Methylene Blue Agar (EMBA), and confirmed with Analytical Profile Index (API) 20E. The resistance against ten antibiotics (nalidixic acid, erythromycin, ampicillin, streptomycin, penicillin G, sulfamethoxazole, ciprofloxacin, tetracycline, amoxicillin, and chloramphenicol) were tested using Kirby Bauer method, based on the standard of Clinical Laboratory Standards Institute (CLSI) in 2012. The result showed that 17 samples were positive containing E. coli (41.5%) and they all resistant to erythromycin, streptomycin, penicillin G, and chloramphenicol (100%), tetracycline (94.1%), ampicillin (76.5%), and nalidixic acid (23.5%). All isolates were also resistant to more three classes of antibiotics which were known as Multi-Drug Resistant (MDR) with mostly pattern of E-AMP-S-P-TE-C (erythromycin, ampicillin, streptomycin, penicillin G, tetracycline, and chloramphenicol).

Keywords | Antibiotic resistant, E. coli, Multi drug resistant, Pork