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Advances in Animal and Veterinary Sciences

Research Article
Adv. Anim. Vet. Sci. 7(9): 776-781
Http://dx.doi.org/10.17582/journal.aavs/2019/7.9.776.781
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Chee Yien Lee1, Chew Yee Tan1, Yi Jing Tan1, Jeffrey Wai Nung Wong3, Daniel Mohan Jacob1, Jia Xin Lim3, Gunaselvi Ananthan3, Siti Suri Arshad2, Gayathri Thevi Selvarajah1, Peck Toung Ooi1*

1Department of Veterinary Clinical Studies, Faculty of Veterinary Medicine, University Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia; 2Department of Veterinary Pathology and Microbiology, Faculty of Veterinary Medicine, University Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia; 3NHK Bioscience Solutions Sdn. Bhd., 4 Lorong Chekor, 3rd mile off Jalan Kelang Lama, 58000 Kuala Lumpur, Malaysia.

Abstract | This paper reports the first nearly complete genome sequence of a Malaysian Porcine bocavirus (PBoV) detected in Malaysia from a porcine organ sample. In Malaysia, both Human bocavirus (HBoV) and PBoV have been reported, at prevalence of 5.6% and 90.9% respectively. Most recently, in 2018, PBoV emerged as a zoonosis when a human case of PBoV-related respiratory infection is reported in a child with history of contact with porcine secretion. With this evidence of human infection with PBoV, considering close contact between humans and pigs in the swine industry, further study of PBoV genetic characterization and epidemiology is warranted. This study attempts to obtain the complete genome sequence of a Malaysian PBoV isolate detected in a pig farm in the state of Selangor. A nearly complete sequence was obtained through primer walking and touchdown PCR method. Based on phylogenetic analysis for the nearly complete genome, the Malaysian PBoV strain is genetically characterized as PBoV G3B, sharing a close relationship with a reference PBoV strain from U.S.A. with 98% homology. This finding may facilitate further epidemiological studies and diagnostic development of PBoV in Malaysia.

Keywords | Porcine bocavirus, PBoV, Pigs, Malaysia