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Advances in Animal and Veterinary Sciences

Research Article
Adv. Anim. Vet. Sci. 7(5): 361-369
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Thair Jawad Kadhim Al-Kelabi1*, Mayada F. Mohamed2, Mohammad Rezaeian3, Hassan Al-Karagoly4

*1,2Department of Public Health, College of Veterinary Medicine, University of Baghdad, Iraq; 3Department of Animal health and Nutrition, Faculty of Veterinary Medicine, University of Tehran; 4Department of internal and treated medicine, College of Veterinary Medicine, University of Al-Qadisiyah, Iraq.

Abstract | This study aimed to evaluate liver growth hormone (cGH) and growth hormone receptor (GHR) gene expression between broilers fed with sweet basil and control groups. One hundred and twenty day-old broiler (Rose) chicks were raised in ground type housing and lasted 42 days. The broiler chicks were divided randomly into 2 treatment groups of 60 birds, each treatment group was further sub-divide into 2 replicates of 30 birds per replicate (the first group fed basal diet without additive as Control and the second group also fed basal diet with Feed additive 0.5 % sweet basil). The broilers were euthanized by cervical dislocation. RNA was extracted from liver, followed by cDNA synthesis and amplification using RT-PCR. Sweet basil supplementation provided better performance (live body weight gain and feed covariation ratio) results. GHR gene expression in the liver was increased not significantly with sweet basil plant supplementation, but cGH gene expression in the liver was significantly higher in chicks fed sweet basil supplementation than in those fed with control diet. cGH mRNA levels in broilers fed sweet basil were greater than control (4.03 vs 0.97 AU). Conclusions: Broilers fed with sweet basil plant, cGH gene expression in the liver where increased significantly (P≤0.05) than in those fed with control diet. Addition of sweet basil plant improved broiler performance by stimulating synthesis and release of growth hormone. Addition SB show increase live body weight gain significantly (P≤0.05) and improved FCR.

Keywords | Sweet basil, Chicken growth Hormone gene, Growth hormone receptor gene, Gene expression, Broilers