Advances in Animal and Veterinary Sciences

Research Article
Adv. Anim. Vet. Sci. 6(12): 526-530
Http://dx.doi.org/10.17582/journal.aavs/2018/6.12.526.530
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Atheer Abdul Razzaq1, Ansam Khalid Mahmood1, Nuhad Mohammed Hammed2, Kifah A. Jasim3, Zaid Saifuldeen Abdulqader3, Harith Jabbar Fahad Al-Mathkhury4*

1College of veterinary medicine, University of Baghdad; 2Al-Anbar education directorate, Ministry of Education; 3Epidemiological Unit, Central public health laboratory, Ministry of Health; 4Department of Biology, College of Science, University of Baghdad, Iraq.

Abstract | The present study was undertaken in order to investigate the role of gentamicin in the gene expression of toxA in Pseudomonas aeruginosa isolated from cow mastitis. A total of ten P. aeruginosa strains originally isolated from cows infected with mastitis. Agar dilution methodology was performed to determine the minimal inhibitory concentration of gentamicin, all of which developed resistance toward gentamicin. The findings presented here demonstrated that all these strains harboured toxA depending on PCR-based assay. Nonetheless, RT-PCR technique revealed a wide variation in expression of toxA. Moreover, the cultivation of P. aeruginosa in the presence of gentamicin, significantly (P< 0.05), induced the expression of toxA, in addition to the possibility of enhancing the virulence of this bacterium. In conclusion, using gentamicin to treat infections caused by P. aeruginosa may participate in more severe outcomes.

Keywords | Gentamicin, Mastitis, Pseudomonas aeruginosa, toxA