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Research Journal for Veterinary Practitioners

Research Article
Res. J. Vet. Pract. 4(4): 71-75
Http://dx.doi.org/10.14737/journal.rjvp/2016/4.4.71.75
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Laleh Yazdanpanah Goharrizi, Shima Tasharofi

Department of Fisheries Science Researches; 2Department of Animal Science Researches, Agriculture and Natural Resources Education and Research Centre, Kerman, Iran.
 
Abstract | Aspergillus flavus from inner part of pistatue was placed on the surface of PDA media culture to produce the spores. Then, different doses (400, 500 and 600 ppm) of savory (Satureja hortensis) essential oil (specifying its component by GC/MS analysis) were added to media to determine its effects as fungal inhibitor. Also, fish feed samples (300 g each) combined with above-mentioned doses of savory essential oil were inoculated with 2 ml spore suspension to evaluate fungal inhibit of savory oil at feed by HPLC method. The extent of the inhibition of fungal growth and aflatoxin (AF) production was dependent on the concentration of essential oil applied. The results indicate that Carvacrol (41.239%) and thymol (20.718%) are the most ingredients of savory oil and use of 500 and 600 ppm savory essential oil in aquatic feeds decrease AF contamination produced by Aspergillus flavus and it could be an appropriate alternative to chemical anti-fungi compounds.

Keywords | Aflatoxin, Carvacrol, Fish feed, Savory, Thymol