Research Journal for Veterinary Practitioners

Research Article
Res. j. vet. pract. 3 (3): 47 - 52
http://dx.doi.org/10.14737/journal.rjvp/2015/3.3.47.52
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Afaf Abdulrahman Yousif*, Mohammed Ali Hussein

Department of Internal and Preventive veterinary Medicine, College of Veterinary Medicine, University of Baghdad, Iraq.

*Correspondence | Afaf Abdulrahman Yousif, University of Baghdad, Iraq; Email: Afaf_a.rahman@yahoo.com

Abstract
This study was carried out to investigate the prevalence of Escherichia coli O157:H7 serotype from diarrheic and non-diarrheic calves. The study was out in Baghdad, a province in Iraq. A total of 350 faecal samples from 35 diarrheic calves and 315 non- diarrheic calves with different ages (up to 1 year) and from both sexes. After initially enrichment, samples were streaked on sorbitol MacConkey agar plus cifixime potassium tellurite (SMA-CT) and Chrom agar™ E .coli O157:H7. Non-sorbitol fermenting (NSF) E. coli isolates were conducted to serotyping using commercial Latex agglutination test for detection of O157 and H7 antigen. E. coli Isolates were additionally tested for virulence factor eae by PCR techniques. Four isolates (11.42%) belonged to E. coli O157:H7 in 35 diarrheic calves and 28 (8.88%) in non- diarrheic calves. All four isolates from diarrheic calves were found positive for intimin (eaeA) gene (100%) and only 13 from 28 isolates (46.42%) were possessing (eaeA) gene in none diarrheic calves. In conclusion, this study revealed the importance of calves to act as a reservoir for E. coli O157:H7. Also, the eaeA genes in a high percentage in most calves suggest that they may be virulent for humans.

Keywords | E. coli O157:H7, Intimin (eaeA) gene, PCR, E. coli O157:H7 in calves