Research Journal for Veterinary Practitioners

Research Article
Res. j. vet. pract. 2 (1S): 11 - 13. Special Issue - 1 (Epidemiology and Occurrence of PPR in Endemic Situations)
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Asma Latif1, Zunaira Akhtar2, Riasat Wasee Ullah1*, Aamer Bin Zahur1, Aman Ullah1, Hamid Irshad1, Adnan Rashid Malik3, Munib Hussain1, Khawar Mahboob4, Shahida Afzal4
1Animal Health Research Laboratories, Animal Sciences Institute, National Agricultural Research Centre Islamabad; 2Department of Clinical Medicine and Surgery, Faculty of Veterinary Sciences, University of Veterinary and Animal Sciences Lahore; 3Department of Animal Husbandry, Muzaffarabad, Azad Government of the State of Jammu and Kashmir: 4Veterinary Research Institute, Lahore
*Corresponding author:

This paper reports the findings of evaluation of Haemagglutination Assay (HA) for detection of Peste des Petits Ruminants (PPR) in faecal samples of sheep and goats persistently infected with PPR. Faecal samples (n=100) collected during an outbreak of PPR were subjected to HA and RT–PCR (gold standard). HA produced more positive results (77/100; 77%) as compared to RT–PCR (29/100; 29%). Kappa analysis indicated no agreement between HA and RT–PCR (kappa = –1.5159). In this study, we found that HA is a non–specific test for detection of PPR Virus (PPRV) in faecal samples of small ruminants, infected with PPRV. Therefore, other sensitive and specific laboratory test should be used for detection of PPRV in faecal samples of persistently infected animals.

Key Words: HA, sensitivity, specificity, PPR