Research Journal for Veterinary Practitioners

Research Article
Res. j. vet. pract. 2 (3): 52 - 54
http://dx.doi.org/10.14737/journal.rjvp/2014/2.3.52.54
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Syed Imam Shah*, Muhammad Abubakar, Muhammad Zaheer, Qurban Ali
National Veterinary Laboratory, Park Road, Islamabad, Pakistan
*Corresponding author: drimamshah@yahoo.com

ABSTRACT
The present study was conducted to evaluate four (04) commercially available (oil based (n=1) and aqueous (n=3) avian influenza vaccines in Pakistan. The vaccines were tested for sterility, safety, inactivation, potency and residual formalin. All the vaccines fulfilled safety and inactivation tests. Residual formalin ranged from 0.12 to 2.89mg/ml against the recommended level of 0.74mg/ml so only one vaccine (locally produced) had a higher quantity of residual formalin than the recommended level. Potency test for vaccine was performed in hundred broiler chicks, comprising of fifteen birds in each group. Locally produced vaccines were found to elicit humeral immune response earlier as compared to the imported vaccine. It was concluded that primary vaccination with locally produced vaccine and booster vaccination with imported vaccine may help in earlier immunization and persistence of antibodies for longer time.

Key Words: Avian influenza, Vaccines, Sterility, Safety, Potency, Inactivation, Broiler, Formalin