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Research Journal for Veterinary Practitioners

Research Article
Res. j. vet. pract. 2 (2): 30 - 35
http://dx.doi.org/10.14737/journal.rjvp/2014/2.2.30.35
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Mohammad Mahmudul Hassan*, Khaled Bin Amin, Md. Ahaduzzaman1, Mahabub Alam2, Md. Shohel Al Faruk, Inkeyas Uddin3
Department of Physiology, Biochemistry and Pharmacology, Faculty of Veterinary Medicine, Chittagong Veterinary and Animal Sciences University, Khulshi, Chittagong-4225, Bangladesh; 1Department of Medicine and Surgery, Faculty of Veterinary Medicine, Chittagong Veterinary and Animal Sciences University, Khulshi, Chittagong–4225, Bangladesh; 2Department of Animal Science and Nutrition, Faculty of Veterinary Medicine, Chittagong Veterinary and Animal Sciences University, Khulshi, Chittagong–4225, Bangladesh; 3Poultry Research and Training centre, Chittagong Veterinary and Animal Sciences University, Khulshi, Chittagong–4225, Bangladesh
*Corresponding author: miladhasan@yahoo.com

ABSTRACT
E. coli and Salmonella are leading cause of illness in layer farms. The emergence of antimicrobial–resistant E. coli and Salmonella are associated with the indiscriminate use of antibiotics in poultry farming. The present study aimed at determination of antimicrobial resistance pattern of E. coli and Salmonella strains isolated from commercial layer from different layer farms under Chittagong district of Bangladesh, during the period of September to December, 2012. Isolation and identification of E. coli and Salmonella were done by using standard methods. A total of 13 isolates of E. coli and 8 isolates of Salmonella were studied. Isolated E. coli and Salmonella were tested for resistance to 10 different antimicrobial agents, using disc diffusion method. The E. coli were found 100% resistant to Tetracycline, Ciprofloxacin, Enrofloxacin and Pefloxacin followed by Amoxicillin (84.62%), Kanamycin (69.24%), Colistin (63.75%), Doxycycline (53.75%) and Neomycin (23.08%). Conversely, E. coli isolates show high sensitivity to Gentamicin (100%) and Neomycin (76.92%). Among the Salmonella isolates, 100% were found resistant to Amoxicillin and Tetracycline followed by Enrofloxacin (87.5%), Ciprofloxacin (87.5%), Pefloxacin (87.5%), Doxycycline (50%), Colistin (50%) and Kanamycin (50%). Salmonella isolates showed high sensitivity (100%) to Gentamicin and Neomycin. All of the isolates showed multiple antimicrobial resistances. Rational use of antibiotics need to be adopt in commercial poultry farming system of Bangladesh to prevent the emergence of drug resistance E. coli and Salmonella. Moreover, Gentamicin might be the drug of choice for both avian colibacillosis and salmonellosis.

Key Words: Antimicrobial, E. coli, Layer poultry, Resistance, Salmonella