Research Journal for Veterinary Practitioners

Research Article
Res. j. vet. pract. 2 (1): 5 - 8
http://dx.doi.org/10.14737/journal.rjvp/2014/2.1.5.8
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Shamim Saleha1*, Abdul Basit1, Kashif Rahim1, Muhammad Shahid2, Mirza Ali Khan2
1Department of Microbiology, Kohat University of Science and Technology (KUST), Khyber Pakhtunkhwa, 26000, Pakistan; 2Microbiology and Biotechnology Center, Veterinary Research Institute (VRI), Khyber PakhtunKhwa, Peshawar, 25000, Pakistan
*Corresponding author: shamimsaleha@yahoo.com

ABSTRACT
In the following study, comparative efficacy of Milk Ring Test (MRT), Serum Plate Agglutination Test (SPAT) was determined by calculating the sensitivities and specificities for detection of Brucellosis in bovines from district Peshawar of Khyber PakhtunKhwa, Pakistan. Considering Polymerase Chain Reaction (PCR) as a gold standard diagnostic test, MRT showed very low sensitivity (4.8%), while its specificity was 90.9 %, likewise, SPAT also showed low sensitivity (41 %), while its specificity was 66.7% in bovines. Statistically, high specificities and low sensitivities of MRT and SPAT in bovines recommended the poor efficacy of these tests, when used individually in contrast to PCR. As PCR is more reliable test, while considering its sensitivity for antigen detection as compare to antibody detection, therefore, PCR may be used as a routine screening test in clinical practices in farms animals for detection of Brucellosis.

Key Words: Bovine brucellosis, MRT, SPAT, PCR, Diagnosis