Research Journal for Veterinary Practitioners

Research Article
Res J. Vet. Pract. 7(4): 83-87
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Tahira Hanif1*, Jawaria Ali Khan2, Aamer Bin Zahur3, Asma Latif3, Aman Ullah3, Muhammad Avais2, Aftab Ahmad Anjum2, Nabeela Haneef4, Muhammad Abdul Rauf Malik2

1Department of Clinical Medicine and Surgery, University of Veterinary and Animal sciences, Lahore, Pakistan; 2University of Veterinary and Animal Sciences, Lahore, Pakistan; 3Animal Health Laboratories, Animal Sciences Institute, NARC, Islamabad, Pakistan, 4PMAS ARID Agriculture University Rawalpindi, Pakistan.

Abstract | Peste des Petits ruminants (PPR) is an acute, extremely fatal and frequently transmissible viral disease of small ruminants. Passive haemagglutination (PHA) and cELISA were conducted for detection of PPR from sera samples at NARC Islamabad. A total number of sera samples n=597 were collected from non-vaccinated goats and sheep. In this study, the results of passive haemagglutination (PHA) were compared against competitive ELISA, as cELISA was considered as gold standard. So, 354 (59.1%) of sera samples were found positive by cELISA and 331(55.3%) were found positive by PHA. However, both tests declared, 292 (48.8%) of sera samples were positive for PPRV antibodies. Kappa value (k=0.654) indicated perfect strong correlation between PHA and cELISA. It was observed that, the sensitivity and specificity of PHA was 82.4% and 84% respectively. In this study, we found that PHA is an economical and rapid serological assay for detection of PPRV antibodies.

Keywords | PHA, sensitivity, specificity, cELISA, Kappa statistics