Advances in Animal and Veterinary Sciences

Research Article
Adv. Anim. Vet. Sci. 4 (4): 209 - 214
Http://dx.doi.org/10.14737/journal.aavs/2016/4.4.209.214
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Muhammad Shoaib1*, Asghar Ali Kamboh1, Abdul Sajid2, Gulfam Ali Mughal3, Riaz Ahmed Leghari4, Kanwar Kumar Malhi1, Shamas-u-Din Bughio5, Akhtar Ali6, Shafiq Alam1, Sajid Khan1, Sardar Ali1

1Department of Veterinary Microbiology, Faculty of Animal Husbandry and Veterinary Sciences, Sindh Agriculture University, Tandojam, Pakistan; 2Veterinary Research Institute, Peshawar, Kpk, Pakistan; 3Department of Livestock Management, Faculty of Animal Husbandry and Veterinary Sciences, Sindh Agriculture University Tandojam, Pakistan; 4Department of Veterinary Medicine, Faculty of Animal Husbandry and Veterinary Sciences, Sindh Agriculture University, Tandojam, Pakistan; 5Department of Veterinary Pharmacology, Faculty of Animal Husbandry and Veterinary Sciences, Sindh Agriculture University, Tandojam, Pakistan; 6Department of Veterinary Pathology, Faculty of Animal Husbandry and Veterinary Sciences, Sindh Agriculture University, Tandojam, Pakistan.

Abstract | The present study demonstrated the prevalence of extended spectrum beta-lactamase (ESBL) -producing enterobacteriaceae in liver samples of commercial broilers (commercial broilers) and backyard chickens (backyard chickens). Results demonstrated that Escherichia coli (E. coli) was the most common isolate recovered from both commercial broilers (44.58%) and backyard chickens (57.03%), that followed by salmonella (commercial broilers: 35.06%; backyard chickens: 21.09%), klebsiella (commercial broilers: 12.98%; backyard chickens: 13.28%), proteus (commercial broilers: 04.76%; backyard chickens: 06.25%), and enterobacter (commercial broilers: 02.59%; backyard chickens: 02.34%). The prevalence of E. coli and salmonella was found higher (P < 0.05) in commercial broilers as compared to backyard chickens. However the prevalence differences of klebsiella between commercial broilers and backyard chickens were found statistically non-significant (P > 0.05).  Among these isolates, 7.76% and 10.95% E. coli isolates were recorded as ESBL -producing from commercial broilers and backyard chickens respectively. While 12.34% and 7.40% salmonella isolates were found positive for ESBL production from commercial broilers and backyard chickens respectively. However, 13.33% klebsiella isolates of commercial broilers were declared as ESBL -producers; whereas klebsiella isolates of backyard chickens and proteus and enterobacter of both commercial broilers and backyard chickens were found negative for ESBL production. These results indicates that microbiota of commercial broilers established a higher number of enterobacteriaceae as compared to backyard chickens, moreover, the prevalence of ESBL -producing enterobacteriaceae in liver of commercial broilers was also higher than backyard chickens.

Keywords | Broiler, Backyard, Enterobacteriaceae, ESBL, Prevalence