Advances in Animal and Veterinary Sciences

Research Article
Adv. Anim. Vet. Sci. 3 (1S): 33 -37. Special Issue-1 (Biotechnological and molecular approaches for diagnosis, prevention and control of infectious diseases of animals)
http://dx.doi.org/10.14737/journal.aavs/2015/3.1s.33.37
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Sandip Kumar Khurana1*, Daya Nand Garg2, Yarvendra Singh2

1National Research Centre on Equines, Hisar, 125 001, Haryana, India; 2Department of Veterinary Public Health, College of Veterinary Sciences, LUVAS, Hisar, 125 004, Haryana, India.

*Correspondence | Sandip Kumar Khurana, National Research Centre on Equines, Hisar, India; Email: sandipkk2003@yahoo.co.in

Abstract
Mycoplasmas are smallest self-replicating prokaryotes and responsible for varied genital and respiratory disease conditions in animals as well as human beings. They assume greater importance due to their resistance for common antibiotics and ability to survive the temperature of cryopreservation during artificial insemination. Mycoplasma equigenitalium has been implicated in reproductive and genital problems of mares and stallions. The conventional cultural methods are quite cumbersome and time consuming. In this study detection rate by capture-ELISA (9.6%) was higher than cultural methods (4.8%) for diseased equines. Overall detection rate by capture-ELISA (3.6%) was also higher than cultural methods (1.8%). Therefore capture-ELISA becomes a simple, rapid and sensitive alternative tool for detection of M. equigenitalium.

Keywords | Mycoplasma equigenitalium, Capture-ELISA