Advances in Animal and Veterinary Sciences

Research Article
Adv. Anim. Vet. Sci. 2 (11): 606 - 611
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Vikrant Jarora, Vinod Kumar Gandotra, Ranjna Sandhey Cheema, Amrit Kaur Bansal, Shahbaz Singh Dhindsa
Department of Veterinary Gynaecology and Obstetrics, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana-141004, Punjab, India.

*Correspondence | Ranjna Sandhey Cheema, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, India; Email:

IgG and IgA class antisperm antibodies were detected in cervical mucus of 25 crossbred cows with SperMar test. Sperm penetration through cervical mucus of these cows was also analysed in vitro. Relationship of in vitro CMPT with IgA and IgG type antibodies in cervical mucus was evaluated. Animals were grouped as G-1(>40% IgG, IgA) and G-II (< 40% IgG, IgA). IgG and IgA type antibodies were detected as 52.7±2.3%, 54.3±9.4% and 28.9±1.4%, 28.5±7.1 in cervical mucus of G-I and G-II, respectively. It indicated a significant (p<0.05) difference of 23.8% and 25.8% in IgG and IgA class antibodies in cervical mucus among the two groups. A total of 54.9%, 56.8% of the tested cows had significant level of IgG and IgA in cervical mucus respectively. Sperm penetration of cattle bull spermatozoa in cervical mucus of 25 cows showed a variation. A mean distance covered by spermatozoa/ 30 min and number of spermatozoa penetrated in peak 0.5 cm in cervical mucus was 15.3±1.8 mm and 356.8±84.9, which ranged from 0-40 mm and 0-1414 respectively. Higher percentage of IgG, IgA did not have negative effect on in vitro CMPT. Out of 25 cows, > 40% IgA and IgG were detected in 13 and 17 cows respectively. Among the cows with higher percentage of IgA/IgG, there were only 6 cows (28%) that had higher level of both IgA and IgG (>40%) and low values of CMPT. There were also one (3.8%) and four (15.3%) cows with only > 40% IgA and IgG and low values of CMPT, respectively. It can be concluded that higher percentage of IgG-ASA and IgA-ASA reduced in vitro penetration of spermatozoa through cervical mucus in 44% of the tested cross-bred cows. However, there was not any negative effect of ASA on in vitro CMPT in a group of cows with > 40% IgG/IgA in cervical mucus.

Keywords | Cross-bred cows, Cervical mucus, IgG, IgA, CMPT