Advances in Animal and Veterinary Sciences

Research Article
Adv. Anim. Vet. Sci. 2 (2S): 39 - 42. Special Issue-2 (Advances in Diagnosis and Control of Infectious Diseases of Animals)
http://dx.doi.org/10.14737/journal.aavs/2014/2.2s.39.42
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Baleshwari Kurmi1, Harshad V Murugkar2, Manu Dixit3, Shanmuga Nagarajan2, Manoj Kumar2, Diwakar D Kulkarni2
1Deptartment of Veterinary Public Health & Epidemiology, College of Veterinary Science & Animal Husbandry, Rewa, Madhya Pradesh, India; 2High Security Animal Disease Laboratory, Bhopal, Indian Veterinary Research Institute, Anand Nagar, Bhopal, Madhya Pradesh, India; 3Deputy Director Veterinary Services, Rewa, Madhya Pradesh, India
*Corresponding author: manu.dixit@live.in

ABSTRACT
Owing to their public health implications, handling of Highly Pathogenic Avian Influenza (HPAI) viruses should be carried out in BSL–3 laboratory inside a biosafety class II cabinet along with recommended personal protection procedures. Treatment with UV light is known to have virucidal activity and is commonly used in most of the laboratories to disinfect the working table. The present study was carried out to check the efficacy of UV light to inactivate HPAI virus (H5N1) inside biosafety cabinet. HPAI virus gets excreted in high concentration in feces of infected birds and these samples are most commonly processed in biosafety cabinets for virus isolation. In this study, fecal samples, wet as well as dry, having known EID50 of virus were exposed to UV light to check its virucidal effect. The results indicated that UV light is unable to inactivate H5N1 virus even after 90 minutes of exposure in dry as well as wet feces. Thus there is a risk of improper disinfection of the biosafety cabinets if UV light alone is used as a source to disinfect the cabinet.

Key Words: HPAI, H5N1, UV light, EID50, Biosafety Cabinets