Advances in Animal and Veterinary Sciences

Research Article
Adv. Anim. Vet. Sci. 1 (1): 35 - 40
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Dilip Singh Barad1, Bharat Singh Chandel1, AbidaliI. Dadawala1, Harshad C. Chauhan1, Hemendra Singh Kher1, Sagar Shroff1, AbidaliGulamhaider Bhagat1, ShoorVir Singh2*, Pravin Kumar Singh2, Ajay Vir Singh2, Jagdip Singh Sohal2, Saurabh Gupta2, Kundan Kumar Chaubey2
1Department of Microbiology, College of Veterinary Science and A.H., Sardarkrushinagar Dantiwada Agricultural University, Sardarkrushinagar - 385 506, Dist. Banaskantha, Gujarat; 2Microbiology Laboratory, Animal Health Division, Central Institute for Research on Goats, Makhdoom, PO -Farah, Dist.- Mathura, 281 122, Uttar Pradesh, India

Study aimed to compare easy to perform field based conventional tests {(fecal smear examination, Delayed Type Hypersensitivity (DTH), AGID} with respect to modern laboratory tests (IS900 PCR and Indigenous ELISA kit) in estimating the incidence of caprineJohne’s disease in two important breeds of goats (Mehsani and Surti) from Gujarat (Western India) in the year 2009. A total of 219 goats screened were categorized as Group-I (123 Mehsani goats), Group-II (76 Surti goats) and Group-III, (20 Non-descript goats). Percent positivity by fecal smear examination (fecal microscopy), Delayed Type Hypersensitivity (DTH), AGID, IS900 PCR and Indigenous ELISA kit was 9.2 (7/76), 21.9 (27/123), 10.9 (24/219), 12.5% (5/40) and 43.3 (95/219), respectively. Rectal pinch smear examination was carried out in 27 DTH positive goats and all smears were negative for the presence of acid fast bacilli. Screening tests (Indigenous ELISA and Delayed Type Hypersensitivity) showed very high incidence of MAP infection in the goat population.

Key Words: Johne’s disease, Mycobacterium aviumsubspecies paratuberculosis, Microscopy, ELISA, IS900 PCR, AGID