Advances in Animal and Veterinary Sciences

Research Article
Adv. Anim. Vet. Sci. 2 (2S): 35 - 38. Special Issue-2 (Advances in Diagnosis and Control of Infectious Diseases of Animals)
Http://dx.doi.org/10.14737/journal.aavs/2014/2.2s.35.38
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Aman Kumar1*, Kanisht Batra1, Arnab Ghosh1, Narender Singh Maan2, Sunayna1, Trilok Nanda1, Sushila Maan1*
1Department of Animal Biotechnology, College of Veterinary Sciences, LLR University of Veterinary and Animal Sciences, Hisar, 125 004, Haryana, India; 2Department of Animal Nutrition, Resource faculty Dept. of ABT, College of Veterinary Sciences, LLR University of Veterinary and Animal Sciences, Hisar, 125 004, Haryana, India
*Corresponding author 1: sushilamaan105@gmail.com; *Corresponding author 2: amankumar34237@gmail.com

ABSTRACT
The aim of the present study was to identify the prevalence of Bovine Herpesvirus–1 (BoHV–1) in bovine bulls using PCR technology as molecular diagnostic tool. A total of 366 biological samples including 162 semen and 204 blood from buffalo bulls and cow bulls collected during July 2010 to December 2013 from different semen banks of Haryana were processed for identification of BoHV–1 using conventional and TaqMan probe based real–time PCR. No semen sample was found positive by gB and gC gene based conventional or real–time PCR for the presence of BoHV–1 DNA, however, only one (0.4%) out of 204 blood samples was found positive for the same. From the study it was concluded that the prevalence of BoHV–1 in Haryana is very less as compared to its surrounding states.

Key Words: Bovine herpesvirus 1, BoHV–1, PCR, gB gene, gC gene, Real time PCR