Advances in Animal and Veterinary Sciences

Research Article
Adv. Anim. Vet. Sci. 2 (2S): 31 - 34. Special Issue-2 (Advances in Diagnosis and Control of Infectious Diseases of Animals)
http://dx.doi.org/10.14737/journal.aavs/2014/2.2s.31.34
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Abdulkarem Al-Shabebi1, Ibrahim El-Sabagh2,3*, Eltayb Abu-Elzein1, Ahmed Zaghawa1, Abdelmohsen Al-Naeem1, Fahdel Housawi1
1Department of Clinical Studies, College of Veterinary Medicine and Animal Resources, King Faisal University, Al-Hufof, 31982, Saudi Arabia.2Central Biotechnology Laboratory, College of Veterinary Medicine and Animal Resources, King Faisal University, Al-Hufof, 31982, Saudi Arabia. 3Department of Virology, Faculty of Veterinary Medicine, Cairo University, Giza, 12211, Egypt.
*Corresponding author:ibrahimelsabagh@yahoo.com

ABSTRACT
The current study was conducted to molecularly identify Sheep pox virus (SPPV) from recent outbreak affected sheep farms in Al-Hassa of Eastern Province of Saudi Arabia during the period between the winter and the spring, 2013. Thirty skin lesions of crusted scab samples were collected from six clinically infected SPPV sheep showing average morbidity and mortality rates of 80% and 15%, respectively. The collected samples were screened for presence of SPPV DNAs using KS-1.5/KS-1.6 and InS-1.1/InS-1.1/-based multiplex PCR. The P32 gene of selected two positive samples was sequenced and aligned with different SPPV, GTPV and LSDV available in GenBank. The phylogenetic analysis revealed that the Sheep pox virus strain; SPPV/Al-Hassa/2013/Saudi Arabia (accession number, KF204447) clustered on SPPV clade with SPPVs from India and China. This finding elucidated that the causative agent of this recent sheep pox outbreak is SPPV.

Key Words: Sheep pox virus, SPPV, P32 gene, molecular detection, sequencing, phylogenetic tree, Al-Hassa, Saudi Arabia.