Advances in Animal and Veterinary Sciences

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Adv. Anim. Vet. Sci. 2 (3S): 16 - 18. Special Issue-3 (Approaches in Diagnosis and Management of Diseases of Livestock and Poultry)
http://dx.doi.org/10.14737/journal.aavs/2014/2.3s.16.18
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Nilofar Yeasmin1, Siddhartha Narayan Joardar1*, Pradip Kumar Das2, Palas Das1, Arkendu Halder1, Sk. Sahanawaz Alam1
1Department of Veterinary Microbiology, West Bengal University of Animal and Fishery Sciences, 68, K. B. Sarani, Post Office–Belgachia, Kolkata– 700037 , India; 2Department of Veterinary Physiology (Rashtriya Krishi Vikash Yojona laboratory), West Bengal University of Animal and Fishery Sciences, 68, K. B. Sarani, Post Office–Belgachia, Kolkata– 700037 , India
*Corresponding author: joardar69@gmail.com

ABSTRACT
With the aim of assessing anti–Newcastle Disease Virus (NDV) antibodies in backyard poultry using indigenously developed dipstick–ELISA tool, blood samples (n= 140) were collected randomly from apparently healthy birds from different districts of West Bengal state and the serum samples were screened subsequently. When assessed by Haemagglutination inhibition (HI) test, 92.86% serum samples were found positive. However, laboratory developed dip–stick ELISA showed 71.43% positivity with the same samples. The indigenously developed test was found primarily useful, however needs validation using large number of samples.

Key Words: Backyard poultry, Dip–stick ELISA, Newcastle disease virus, Seromonitoring