Advances in Animal and Veterinary Sciences

Research Article
Adv. Anim. Vet. Sci. 9(12): 2258-2265
Http://dx.doi.org/10.17582/journal.aavs/2021/9.12.2258.2265
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Nabila Elmiranda1, Andi Hiroyuki1,2*, Vanessa Ayu Sumirat3, Neni Anggraeni3, Alkaustariyah Lubis4, Rini Widyastuti4,5, Mas Rizky A.A Syamsunarno2,5

1Veterinary Study Program, Faculty of Medicine, Universitas Padjadjaran, Jatinangor, Indonesia, 45363; 2Department of Biomedical Sciences, Faculty of Medicine, Universitas Padjadjaran, Jatinangor, Indonesia, 45363; 3Medical Laboratory Technologist, Bakti Asih School of Analyst, Bandung, 40192, Indonesia; 4Laboratory of Animal Reproduction and Artificial Insemination, Department of Animal Production Animal Husbandry Faculty, Universitas Padjadjaran, Jatinangor, Indonesia, 45363; 5Central Laboratory, Universitas Padjadjaran, Jatinangor, Indonesia, 45363.

Abstract | Vasectomy is a male contraceptive method considered effective and safe because it is performed under local anesthesia where ductus deferens end up in ligation through a small scrotal incision. However, the effect of short-term period after vasectomy has not been well studied. This study aimed to determine the effect of short-term period after vasectomy on hematological profile and sperm quality. This study used 13 mice (Mus musculus) BALB/c strains which were divided into two groups, namely control group (Sham) and treatment (vasectomy). After 14 days, blood sampling was performed for hematology profile examination, and spermatozoa had been collected from cauda epididymis for analysis of spermatozoa quality. The results showed that the average value of erythrocyte count, hemoglobin, and hematocrit were significantly increased 21%, 14.77 %, and 18.28 % respectively in the treatment group, and mean corpuscular hemoglobin (MCH) decreased in the treatment group (p<0.05). The monocyte percentage parameter was significantly decreased in the treatment group compared to the control group (p<0.05). An increase in spermatozoa concentration occurred in the treatment group while spermatozoa motility decreased. Changes in hematological values ​​were still within normal limits and there were no health problems. Differences in hematological values ​​are caused by changes in nutrition and stress conditions after vasectomy surgery. In addition, vasectomy caused a decrease in sperm quality in mice.

Keywords | Vasectomy, Hematological profile, Spermatozoa quality, Mice, Cauda epididymis