PCR amplification of genomic DNA sampling carried out for pch-1 DNA region using specific primers DMTF CHSF1 and DMTFCHSR1.
Lane1-2: M. canis from cat Samples, Lane 3: M. canis from dog samples, Lane 5: M. canis from human sample. B, ITS1 specific primer yielded a 700bp. 

Phylogenetic analysis of CHS1 and ITS. Sequence data representing the M. canis isolated from examined pets. The bar representing the genetic distance is drawn to scale. The single asterisk represents sequence that determined in this study. The other sequences were obtained from Gene Bank.