Advances in Animal and Veterinary Sciences

Research Article
Adv. Anim. Vet. Sci. 9(12): 2201-2215
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Rasha H. Elsabagh 1,2, Haithem A. M. Farghali3*, Ibrahim A. Emam3, Eman Ragab2, Abdelfattah A. Nada4, Salah A. Selim2*

1Animal Health Research Institute (AHRI), Shibin El-Kom Lab, Agriculture Research Centre, 32518 El-Monofiya, Egypt; 2Department of Microbiology, Faculty of Veterinary Medicine, Cairo University, 12211 Giza, Egypt; 3Department of Surgery, Anesthesiology and Radiology, Faculty of Veterinary Medicine, Cairo University,12211 Giza, Egypt; 4Animal Health Research Institute (AHRI), Agriculture Research Centre, 12618 Dokki, Egypt.

Abstract | The upsurge need for a rapid and accurate prognostic system for feline mammary neoplasia is crucial, as almost 90% of mammary tumors in cats tend to metastasize. Here, appears the importance of circulatory cancer biomarkers (liquid biopsies) such as circulating tumor cells (CTCs) and their subpopulation circulating cancer stem cells (cCSCs) as an effective, feasible, repeatable, and non-invasive tool for tumor prognosis and treatment monitoring. In the current prototype study, we detected and enumerated the circulatory breast cancer stem cells (BCSCs) for the first time in the feline peripheral blood by using the flow cytometer analysis (FCA) against their specific cellular markers (CD44+/CD24- and CD133+). In addition, we calculated Area Under the Curve (AUC), like hood ratios and cutoff points by statistical analysis. The results revealed statistically significant differences (p <0.0001) between healthy and diseased animal groups with an excellent AUC value (0.902 for CD44+/CD24- cells and 0.990 for CD133+ population) that supported the use of FCA as a sensitive, specific, and rapid diagnostic and monitoring tool for mammary carcinoma. We also set up a cutoff value of diagnostic significance (>276 for CD44+/CD24- populations and >12 for CD133+ cells).

Keywords | Feline mammary tumor; Cancer Biomarkers; Tumor Stem Cells; CD133+; CD44+/CD24-, Flow cytometer.