Advances in Animal and Veterinary Sciences

Research Article
Adv. Anim. Vet. Sci. 9(12): 2116-2123
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Ayatollah I Bassiouny, Kafafy MH, Heba M El Naggar*

Veterinary Serum and Vaccine Research Institute (VSVRI), Agriculture Research Center (ARC),El-Seka-Beda Street, Abbasia, 131, Cairo, Egypt.

Abstract | The current study was conducted to evaluate the adjuvanted aerosol vaccination of pigeon pox vaccine (PPV) with Gel 01 in comparison with feather follicle non adjuvanted route of vaccination. In order to determine the best aerosol field dose of PPV, challenge was done for 3 groups of pigeon (group no 2, 3 and 4) aerosolly vaccinated with non adjuvanted prepared live attenuated Vero cell tissue culture adapted PPV different field dose ( Log 10 2,5, 3.5, and 4.5 TCID50) the protection percent were 75%, 80% and 80% respectively as field dose of 10 3.5TCID50 was the field dose of choice to be used with different concentrations of Gel 01 adjuvant. Four groups of pigeon were vaccinated with PPV; group (1) vaccinated via feather follicle route in addition to other 3 groups of pigeon ( group 5, 6 and 7) vaccinated via aerosol route with different Gel 01 adjuvant ( 5%, 10% and 15% respectively ). Serum samples were collected weekly for 6 weeks. The highest level of induced antibodies detected by virus neutralization test (VNT) and ELISA were (3.0, 2.0, 2.5 and 2.5 NI respectively) and ( 1.9, 1.5, 1.8 and 1.7 S/P respectively) at the 3rd week post vaccination. The level of antibodies reduced dramatically in pigeon groups vaccinated via aerosol route group (5, 6 and 7) reached (1.5, 1.75 and 2.0 NI respectively ) and (1.2 , 1.3 and 1.4 S/P respectively ) at 6th week on contrast to group (1) vaccinated with feather follicle route has a slight decrease in antibody level reached 2.75 NI and 1.8 S/P at the 6th week post vaccination. Negative result for VNT and ELISA were found in control non vaccinated group all over the period of study. All vaccinated four pigeon groups and non vaccinated control group were challenged by inoculation of the virulent PPV after 3 weeks from vaccination showing protection percent of 95%, in group (1) vaccinated by feather follicle while it was 85%, 90%and 90% in groups vaccinated via aerosol route with different Gel 01 percent ( 5%, 10% and 15% respectively ) showing that 10 % Gel 01 is the concentration of choice to be used with PPV via aerosol route. On contrast, the control non- vaccinated pigeons showed 0% of protection. This study recorded the efficacy of aerosol vaccination of tissue culture adjuvanted PPV in pigeon using Gel 01 comparison to usual routine vaccination with unadjuvanted PPV by feather follicle route to establish a more safe route of vaccination in valuable oriental and racing pigeons and easier route of vaccination of large pigeon flock with recommendation of booster vaccination after 3 weeks in order to maintain a high level of protection and more duration of immunity.


Keywords | Pigeon Pox, Aerosol, Gel 01, ELISA , Vaccine.