Advances in Animal and Veterinary Sciences

Research Article
Adv. Anim. Vet. Sci. 9(12): 2069-2076
Http://dx.doi.org/10.17582/journal.aavs/2021/9.12.2069.2076
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Zaghloul, R.A.1, Solafa A. Ismail2, Gamal Enan3*, Rasha M. El-Meihy1, Abdel-Rahman H.M.1

1Agricultural Microbiology Department, Faculty of Agriculture, Benha University, Moshtohor, Kaluybia, 13736, Egypt; 2Faculty of Technology and Development, Zagazig University, Egypt; 3Faculty of Science, Zagazig University, Egypt.

Abstract | This research was carried out to evaluate the bio-ethanol production by yeasts. Out of the 7 tested yeast isolates, only 5 isolates were able to produce bio-ethanol efficiently during the fermentation of sugarcane molasses. It is worthily to mention that the highest bio-ethanol producers from sugarcane and sugar beet molasses were Saccharomyces cerevisiae F15 (S. cerevisiae) followed by Candida kefyr EMCC77 (C. kefyr). Therefore, they were selected for further experiments. Regarding the effect of environmental factors on bio-ethanol yield, the optimum pH values for bio-ethanol yield were 4.5 and 5 for S. cerevisiae and C. kefyr respectively. Whereas the optimum incubation temperature for bio-ethanol production for both S. cerevisiae and C. kefyr was 32oC. Maximum production of bio-ethanol by S. cerevisiae and C. kefyr was optimally obtained after 48hours incubation period. Under all optimum conditions, S. cerevisiae gave bio-ethanol yield from sugar cane molasses higher than that obtained by C. kefyr and vice versa in case of sugar beet molasses. Finally, immobilized cell technique showed better results in bio-ethanol production than free cells. In view of the obtained results, it could be recommended that the S. cerevisiae and C. kefyr can be used for industrial production of bio-ethanol.

Keywords | Bio-ethanol, Biofuels, Immobilization, Fermentation, Sugarcane molasses, Sugar beet molasses, Saccharomyces cerevisiae, Candida kefyr EMCC77