Advances in Animal and Veterinary Sciences

Research Article
Adv. Anim. Vet. Sci. 9(11): 1919-1924
Http://dx.doi.org/10.17582/journal.aavs/2021/9.11.1919.1924
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Widi Nugroho1*, Karlina1, Sarah Maghfirah1, Muhamad F.A. Rasyid1, Firdauzi O. Purwanto1, Muhammad A. Lesmana2, Siska Aditya3

1Laboratory of Veterinary Public Health, Faculty of Veterinary Medicine, Universitas Brawijaya, Kalisongo, Dau, Malang, East Java, Indonesia, 65151; 2Laboratory of Veterinary Surgery, Faculty of Veterinary Medicine, Universitas Brawijaya, Kalisongo, Dau, Malang, East Java, Indonesia, 65151; 3Laboratory of Veterinary Biochemistry, Faculty of Veterinary Medicine, Universitas Brawijaya, Kalisongo, Dau, Malang, East Java, Indonesia, 65151.

Abstract | This study aimed to produce rabbit’s anti-pig serum polyclonal antibodies, as part of an attempt to develop a rapid, reliable, and self-administered test for detecting pork adulteration in meat products. The pig antigen for the antibody production was obtained by the treatment of pig serum using 10% caprylic acid. Experimental immunization of rabbits using the pig antigen demonstrated that antibodies were detectable on day 56 post-initial immunization. The antibodies were capable of detecting pork juice at as low as 1% dilution. Further, the antibodies reacted specifically to pork amongst juices of pork, beef, lamb, goat, duck, and catfish meat species tested. This study provides extended knowledge for the development of a rapid, and self-administered test for detecting pork adulteration in meat products.

Keywords | Pork adulteration, Pig antigen, Caprylic acid, Rabbit’s anti-pig serum polyclonal antibodies, Agar gel immunodiffusion