Advances in Animal and Veterinary Sciences

Research Article
Adv. Anim. Vet. Sci. 9(11): 1876-1885
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Aleksandr Lysenko1*, Vitaliy Mikhalev1, Pavel Parshin2, Vladimir Safonov3, Vladimir Skorikov1, Sergey Shabunin2

1Laboratory of Reproductive Organs, Mammary Gland and Young Farm Animals Diseases, FSBSI “All-Russian Veterinary Research Institute of Pathology, Pharmacology and Therapy”, Voronezh, Russia; 2Department of Pharmacology, FSBSI “All-Russian Veterinary Research Institute of Pathology, Pharmacology and Therapy”, Voronezh, Russia; 3Joint Research Laboratory of Fundamental and Applied Problems of Biogeochemistry and Veterinary Medicine of the Volga-Caspian Region of Astrakhan State University and Vernadsky Institute of Geochemistry and Analytical Chemistry, Astrakhan State University, Moscow, Russia.

Abstract | The article describes the study on the efficacy of recombinant interferons in the restoration of functional activity of the ovaries. The search for rational means of restoring gonadal hormonal function is relevant given the high prevalence of hypofunctional disorders in highly productive cows. Ovarian hypofunction was found to occur in 28.9-43.9% of cows examined and increase with higher milk productivity. In first-calf heifers, ovarian hypofunction was detected 1.3 to 2.0 times more frequently than in second-lactating cows and 1.3 to 2.8 times more frequently than in third- or later-lactating cows. Complex use of bovine recombinant interferons -α, -γ twice with a 24-hour interval at a dose of 5.0 ml and interferon-tau 8-9 days after the second injection of interferons -α, -γ twice with an interval of 24 hours at a dose of 10.0 and 5.0 ml is accompanied by an increase in therapeutic efficacy for cows with ovarian hypofunction by 10.0-20.0% (90.0%). After the complex use of bovine recombinant interferons -α, -γ and interferon-tau on days 18-20 after the treatment onset, the content of glucose increased by 55.5%, that of vitamin A - by 67.2%, vitamin C - by 14.1 %, serum bacterial activity - by 5.0%, serum lysozyme activity - by 13.3%, phagocytic activity of leukocytes - by 6.2%, estradiol - by 36.7%, progesterone - by 5.5 times, dehydroepiandrosterone sulfate - by 73.1 %. The dynamics of the blood anti-Müllerian hormone increase in cows on days 18-20th after the treatment onset slows down (25.2%) as follicles reach preovulatory condition. Apart from restoring functional ovarian activity, recombinant interferons facilitate the stabilization of energy metabolism, the weakening of lipid peroxidation and endogenous poisoning, and the activation of antioxidant protection.

Keywords | Cows, Cytokines, Hormones, Ovarian hypofunction, Recombinant interferons