Advances in Animal and Veterinary Sciences

Research Article
Adv. Anim. Vet. Sci. 9(11): 1844-1850
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Salama Rehab1,2*, Akira Kubota2, Yusuke Kawai2, Ahmed Ibrahim3, Dina M.W. Shibat El-Hamd4, Nabila Osman3

1Department of Poultry Diseases, Faculty of Veterinary Medicine, Aswan University, 81528, Egypt; 2Department of Veterinary Medicine, Obihiro University of Agriculture and Veterinary Medicine, 2-11 Inada, Obihiro, Hokkaido, 080-8555, Japan; 3Department of Poultry Diseases, Faculty of Veterinary Medicine, South Valley University, Qena, 83523, Egypt; 4Department of Poultry Diseases, Qena Regional Laboratory, Animal Health Research Institute, Agriculture Research Center (ARC), Qena, 83523, Egypt.

Abstract | Intensive usage of antibiotics in the poultry sector has become a major area of concern with the consequent emergence of antibiotic-resistant bacteria (ARB) and accordingly their access into the food chain, thus threatening the human health. In the current study, A total of 250 specimens from different local hatcheries of Luxor province (southern Egypt) have been examined for the incidence of Staphylococcus aureus using standard microbiological methods and biochemical tests. The overall prevalence was 27.60% (n = 69/250). Only 30 isolates have been screened for in vitro drug sensitivity bioassay against 18 antimicrobial discs. Our findings revealed that all isolates showed high resistance against β-lactams, including oxacillin (83.33%, n= 25/30), ampicillin (80.00%, n= 24/30), penicillin (73.33%, n= 22/30), and amoxicillin (60.00%, n= 18/30). On the other hand, the highest susceptibility was shown for vancomycin (93.33%, n= 28/30), trimethoprim sulpha methoxazole (80.00%, n= 24/30), and chloramphenicol (70.00%, n = 21/30). Results from a pathogenicity study conducted on 300 one-day-old chicks (SPF) manifested the highest mortality rate of 34.00% in one infected group, and most deaths were in the first three days of all infected groups. Based on the current study, constant monitoring of antibiotic susceptibility phenotypes is deemed prudent and necessary for evaluating the utility of certain antibiotics for treating S. aureus infections. Additionally, any alteration of resistance over the years should be identified, and all centers in charge must determine their own resistance profiles, as reducing the rate of antibiotic resistance will contribute to reducing the cost of treatment. Such measures can lead to improvement of the poultry industry, which represents a vital source of economy in Egypt.

Keywords | Baby chicks, Pathogenicity index, Prevalence, Sensitivity bioassay, S. aureus