Advances in Animal and Veterinary Sciences

Research Article
Adv. Anim. Vet. Sci. 9(11): 1810-1815
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Nasser Ghanem1,2*, Romysa Samy1, Beshoy SF Khalil2, Ibrahim Abdalla Hassan Barakat3,4, Ahmed Yousry Sayed Ahmed5, Esraa Moheb Ahmed Ismail5, Ayman A. Diab5, Gehan Safwat5, Md. Fakruzzaman6, Il-Keun Kong7

1Department of Animal Production, Faculty of Agriculture, Cairo University, Giza, Egypt; 2Cairo University Research Park (CURP), Faculty of Agriculture, Cairo University, Giza, Egypt; 3Zoology Department, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Kingdom of Saudi Arabia; 4Cell Biology Department, National Research Center, 33 Bohouth St., Dokki, Giza, Egypt; 5Faculty of Biotechnology, October University for Modern Sciences and Arts– MSA University, Giza, Egypt; 6Department of Genetics and Animal Breeding, Patuakhali Science and Technology University, Out Campus, Khanpura, Babuganj, Barishal-8210, Bangladesh; 7Division of Applied Life Science (BK21), Graduate School of Gyeongsang National University, Jinju 660-701, Republic of Korea.

Abstract | In vitro embryo production is a well-known biotechnology tool to improve and sustain animal productivity. Therefore, optimization of this technique would enhance both animal productivity and farm profitability. The aim of the present study was to compare the mitochondrial activity and patterns of expression of genes that contribute to its regulation during the in vitro maturation of buffalo (Bubalus bubalis) and bovine (Bos indicus) oocytes. Ovaries were collected from local Egyptian abattoirs and cumulus-oocytes complexes (COCs)were aspirated from 2-8mm follicles diameter and were divided into four categories according to oocyte morphology. The grade A and grade B were cultured in TCM medium (supplemented with all required chemicals and hormones) for 22 hours at 38.5o C and collected after their in vitro maturation (IVM). The total RNA of the oocytes was then extracted and target mitochondrial transcripts (TFAM and CPT2) were analyzed by real-time PCR. The results of this work revealed the intensity of mitochondria and lipids was reduced in good than bad matured bovine oocytes. However, there was no change of mitochondrial and lipid fluorescent intensities of bad quality oocytes before and after in vitro maturation. The expression profile of CPT2 gene was higher in immature compared to matured oocytes of bovine while, buffalo oocytes did not shown differences in the expression of this gene. Furthermore, the expression profile of CPT2 gene was lower in immature and matured buffalo oocytes than those of bovine. The transcript abundance of TFAM did not indicate any differences among in vitro maturation of both species. It was concluded that the patterns of the gene expression of CPT2 vary during in vitro maturation of bovine oocytes in reflecting their maturation competence than that of buffalo. Increased metabolic activity of oocytes during IVM is in line with CPT2 expression that is involved in lipid oxidation required for this process.

Keywords | Oocyte quality, Gene expression, mRNA, IVM