Advances in Animal and Veterinary Sciences

Research Article
Adv. Anim. Vet. Sci. 9(11): 1781-1790
View Full HTML
Download PDF

Eko Prasetyo Nugroho1, Agus Setiyono2*, Upik Kesumawati Hadi3, Wiwin Winarsih2, Dwi Astuti4

1Graduate School, Animal Biomedical Science Study Program, Faculty of Veterinary Medicine, IPB University, Bogor, Indonesia; 2Division of Veterinary Pathology, Faculty of Veterinary Medicine, IPB University, Bogor, Indonesia; 3Division of Veterinary Parasitology and Entomology, Faculty of Veterinary Medicine, IPB University, Bogor, Indonesia; 4Biology Research Center, Division of Zoology, Indonesian Institute of Sciences, Bogor, Indonesia.

Abstract | This study aimed to determine the characteristics, distribution and diversity of nuisance flies in dairy farms at Ampel and Mojosongo Districts, Boyolali Regency. The Q fever incidence in dairy cattle and the nuisance flies potency as vectors of Q fever were also examined, considering that previous studies found positive cases of Q fever in 4 beef cattle slaughtered at the Ampel Slaughterhouse, Ampel District, Boyolali Regency. The collection of flies was carried out using the sweep net and the Nzi Trap. A total of 351 nuisance flies were collected, analyzed, and as many as 200 flies were examined by nested-PCR with OMP1, OMP2, OMP3 and OMP4 primer. We also collected and tested milk samples (20 samples) with nested-PCR. The results showed no amplified DNA band (437 bp) in both milk and fly samples. It indicated that there is no evidence of pathogenic infection causing Q fever in dairy cattle at Boyolali Regency and the nuisance flies have not been shown to act as a vector of C. burnetii.

Keywords | C. burnetii, Q fever, Nuisance flies, Dairy cattle, Boyolali regency