Advances in Animal and Veterinary Sciences

Research Article
Adv. Anim. Vet. Sci. 9(10): 1684-1691
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Basant M. Mahmoud1, Sana’a O. Ebrahim1*, Mohamed A. Kandeil2

1Biochemistry Department, Faculty of Science, Beni-Suef University, Beni-Suef, Egypt; 2Biochemistry Department, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef, Egypt.

Abstract | Rheumatoid arthritis is characterized by amplification of pro-inflammatory cytokines, white blood cells, and platelets contributing to acute edema and hyperalgesia. The aim of this study is to evaluate the enhancing influence of extra virgin olive oil (EVOO) and Evening primrose oil (EPO) in adjuvant-induced arthritic rat models and compare between EPO and EVOO effects. A subcutaneous injection of 200 µl Freund’s complete adjuvant into footpad of the right hind leg of male Wistar rats at two consecutive days to induce Rheumatoid arthritis. Arthritic rats were treated orally with Extra Virgin olive oil and Evening Primrose Oil at doses of 5 mg/kg. b. wt./day for 10 and 21 days and then compared to corresponding arthritic control at the same periods. Blood sample collection, scarification of rats, and preparation of ankles and spleen for histopathological investigations were carried out at the end of each treatment period. Statistical analysis was carried out using Graph Pad Instat software (version 8, ISS-Rome, Italy). The results showed edema at the right hind limb a few hours after CFA injection. EVOO and EPO treatments ameliorated the elevated ankle diameter, white blood cells count and platelets number in arthritic rats. The Treatments decreased mitotic figures and lymphoblasts activation in spleen specimens. Enhanced inflammatory biomarkers (rheumatoid factor, tumor necrosis factor-alpha, interleukin-1beta, and interleukin-6) levels in arthritic rats were markedly decreased due to EVOO and EPO treatments administration. Conclusion: EVOO and EPO had significant anti-inflammatory effects on arthritic rats. EVOO and EPO treatments lead to a significant improvement of blood cell count, histological changes, and augmented inflammation markers. More studies on EVOO and EPO combination with other Disease-modifying antirheumatic medications are recommended to increase their effectiveness with less toxicity.


Keywords | Rheumatoid arthritis, Cytokines, Autoimmunity, Interleukins, Extra virgin olive oil (EVOO), Evening primrose oil (EPO)