Advances in Animal and Veterinary Sciences

Case Report
Adv. Anim. Vet. Sci. 9(10): 1559-1563.
Http://dx.doi.org/10.17582/journal.aavs/2021/9.10.1559.1563
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Sri Hartati1, Tri Untari2, Annisaa Lulu Nuraini3, Alfarisa Nururrozi1*

1Departement of Internal Medicine, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Yogyakarta, Indonesia; 2Departement of Microbiology, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Yogyakarta, Indonesia; 3Postgraduate Student, Veterinary Science, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Yogyakarta, Indonesia.

Abstract | In recent years, some outbreaks of skin lesions suspected to be avian pox were observed in a farms layer chicken in Yogyakarta Province, Indonesia. This article reports a confirmed case of avian pox virus from three-layer chickens and a pigeon kept in same farm area. Both chickens and pigeon showed nodular lesions on unfeather head skin and fibro-necrotic lesions on the mucus membrane of the oral cavity. Avian pox virus was propagated from nodular and fibro-necrotic lesions by inoculation into the chorioallantoic membranes (CAM) of 10-days-old specific-pathogen-free chicken embryos for 5-7 days. The CAM was examined to observe the pock lesions. The tissue samples from nodular lesions were stained with Hematoxylin-Eosin stain for histopathological analysis. For PCR, after DNA extraction, a 578-bp fragment of avian pox virus from 4b core protein gene was amplified. The data were analyzed descriptively. Both chickens and pigeon samples were positive for avian pox virus on propagation, histopathological, and PCR examination, respectively. The conclusion of this report there is potential transmission of avian poxvirus between chickens and pigeon, so other birds should not be kept inside the farm.

Keywords | Avian pox virus, Chicken, Pigeon, PCR, Histopathology