Advances in Animal and Veterinary Sciences

Research Article
Adv. Anim. Vet. Sci. 9(10): 1553-1558
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Rondius Solfaine1, Lailatul Muniroh2*, Sadarman3, Apriza4, Agung Irawan5

1Department of Pathology, Faculty of Veterinary Medicine, University of Wijaya Kusuma Surabaya, Jl. Dukuh Kupang XXV/54, Surabaya, 60225, Indonesia; 2Department of Nutrition, Faculty of Public Health, Universitas Airlangga, Kampus C UNAIR, Mulyorejo, Surabaya, 60115, Indonesia; 3Department of Animal Science, Sultan Syarif Kasim State Islamic University, Pekanbaru, Indonesia; 4Nursing Department, Faculty of Health Sciences, Pahlawan Tuanku Tambusai University, Kampar, 28412, Indonesia; 5Vocational Program of Animal Husbandry, Universitas Sebelas Maret, Surakarta 57126, Indonesia.

Abstract | Recently, food consumption has changed following to sedentary lifestyle and high purine diets. This study examined the anti-inflammatory effect of Coleus amboinicus leaves extracts on uric acid-induced nephrotoxicity rats. Twenty-four Wistar rats were distributed into 3 groups (n = 8 rats for each group). The experiment was performed based on the following treatment: P0= rats received normal saline after being fasted for 12 h; P1= rats were induced with 500 mg/kg BW uric acid, 750 mg/kg BW oxonic acid, and 0.1% sodium carboxymethyl cellu­lose (CMC-Na) (positive control); and P2= induced-rats similar to P1 but received 500 mg/kg BW CA plant extract. The experiment lasted for 14 days. All rats were sacrificed for blood samples and tissue fixation on day 15 and were analyzed for serum creatinine and blood urea nitrogen (BUN), glutathione peroxidase (GPx), and transforming growth factor-β1 (TGF- β1) concentrations. Kidney tissues were collected for immunohistochemical and hematoxylin-eosin staining. Results showed that induction of uric acid (P1 and P2) revealed necrotic lesions in the tubular membrane. Concurrently, serum creatinine and BUN concentration increased (p<0.05) in the P1 group while the P2 can compensate for such increasing trends, giving a similar value to P0 (p>0.05). Treatment with CA leaves extract at 500 mg/kg BW (P2) increased GPx and TGF-β1 concentrations (p<0.05) compared with the positive control group (P1). Immunohistochemical analysis showed an increase in the expression of CD-68 and CD-163 macrophages characterized by brownish aggregate in the area of renal tubules and glomerulus in the positive control and treatment groups compared to the control group. This evidence suggests that Coleus amboinicus leaves extract can be used as an anti-inflammatory agent because it was effective to inhibit acute kidney failure by restore BUN and serum creatinine to normal concentrations.

Keywords | Anti-inflammatory agents, Coleus amboinicus, Uric acid, Toxic chemicals