Advances in Animal and Veterinary Sciences

Research Article
Adv. Anim. Vet. Sci. 9(10): 1547-1552
Http://dx.doi.org/10.17582/journal.aavs/2021/9.10.1547.1552
View Full HTML
Download PDF

Nurina Titisari1*, Tiara Widyaputri2, Oktalita Kusumawati3, S. Prasetyawan4

1Department of Physiology, Faculty of Veterinary Medicine, Brawijaya University, Malang, Indonesia; 2Department of Clinical Pathology, Faculty of Veterinary Medicine, Brawijaya University, Malang, Indonesia; 3Faculty of Veterinary Medicine, Brawijaya University, Malang, Indonesia; 4Departement of Chemistry, Faculty of Mathematics and Natural Sciences, Brawijaya University, Malang, Indonesia.

Abstract | This study aimed to determine the preventive effect of Sumbawa horse milk (SHM) on elevated IL-6 expression and the inflammatory cells in the duodenum of Inflammatory Bowel Disease (IBD) animal models. As an IBD animal model, 20 white male rats (Rattus norvegicus) Wistar strain were divided into five groups, i.e. T1 group (negative control), T2 group (positive control), and Sumbawa horse milk groups with different dose of administration that were T3 (0.5 mL/rat), T4(1 mL/rat), and T5 (1.5 mL/rat). The IL-6 expressions level was obtained by flow cytometry method from duodenal tissue, while the number of inflammatory cells was determined by duodenal histopathology examination. Data analysis in this study was quantitative data analysis which used statistical tests with One-Way ANOVA (α 95%) and continued with Tukey’s Test if significant difference was found. The results showed that an increase in IL 6 expression in all groups. The same thing was seen in the results of the inflammatory cell count, showed decrease in all groups. The conclusion in this research was SHM therapy at a dose of 1 mL (T4) and 1.5 mL (T5) could prevent the increase of IL-6 level and elevation of inflammatory cells in the duodenum of rats compared to the positive control group (T2).

Keywords | Lactoferrin, Lysozyme, Mare, Rats, Rattus norvegicus