Advances in Animal and Veterinary Sciences

Research Article
Adv. Anim. Vet. Sci. 9(10): 1532-1546
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Ahmed F. Abou-Elnaga1, Gaber E. Batiha2, Christian T. Potter3, Ibrahim F. Rehan4*

1Department of Husbandry and Development of Animal Wealth, Faculty of Veterinary Medicine, Mansoura University, Egypt; 2Department of Pharmacology and Therapeutics, Faculty of Veterinary Medicine, Damanhour University, Damanhour, Al Beheira, Egypt; 3Department of neuroscience, center for neuroscience at the University of Pittsburgh, Pennsylvania, United States of America; 4Department of Husbandry and Development of Animal Wealth, Faculty of Veterinary Medicine, Menofia University, Shebin Alkom, Menofia, Egypt.

Abstract | Anxiety-related behaviors are indices reflecting the welfare conditions of animals. Correlating anxiety levels of C57BL/6 and DBA/2 in different tests to glycomic responses as a realistic biochemical markers in mice remains unclear. Sixty-four mice, 12 weeks old, were housed either singly or in groups of four (n= 32 each) and tested for anxiety-related behavior after 7 weeks of different housing and their anxiety levels were correlated to their functional glycomic responses in the context of different housing conditions. DBA/2 mice demonstrated less grooming, lower incidence of rearing, higher defecation, and a higher latency to begin rearing and grooming in an induced grooming test. They also displayed more anxiety-related behaviors in DL exploration than the C57BL/6 mice did. The serum N-glycans were examined using glycoblotting along with matrix-assisted laser desportionionization-time of flight/mass spectrometry (MALDI-TOF/MS) to follow the molecular correlates of anxiety in mice behaviors in-vivo. Sialylated N-glycan was significantly increased in the DBA/2, and correlated with the mice’s behavioral data, confirming that there were more anxiety-like responses than the C57BL/6 mice. To our knowledge, sialylated N-glycan is a novel biomarker of anxiety. This approach has the potential to improve neurobehavioural research on anxiety.

Keywords | Anxiety, N-glycan, MALDI-TOF/MS, Welfare, Mice