Advances in Animal and Veterinary Sciences

Research Article
Adv. Anim. Vet. Sci. 9(10): 1525-1531
Https://dx.doi.org/10.17582/journal.aavs/2021/9.10.1525.1531
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Samar S. Ewies1*, Mahmoud Samir2, Abdelsatar A. Arafa2, Sabry M. Tamam1, Hanafy M. Madbouly1

1Virology Department Faculty of Veterinary Medicine, Beni-Suef University, Egypt; 2Reference Laboratory for Veterinary Quality Control on Poultry Production, Animal Health Research Institute, Agriculture Research Center, Giza, Egypt.

Abstract | In the present study, the antiviral activity of Curcumin (Curcuma longa) and bottle gourd (Lagenaria siceraria) extracts was evaluated against the field and vaccine strains of Marek’s disease virus (MDV). Plaque reduction assays were performed and the obtained results showed that both Curcumin and bottle gourd had a reduction effect on the number of plaques in comparison with the control vaccines. Curcumin exhibited prominent antiviral effect expressed as 80% and 66% plaque reduction percent (PR%) against HVT and Rispen’s respectively at the 1:8 virus dilution. While, bottle gourd has moderate antiviral action at 1:4 dilution; it has 40% and 33% PR% against HVT and Rispen’s respectively at dilution 1:8. The obtained results were evaluated using polymerase chain reaction PCR SYBR Mix and showed antiviral activity of these plants against Marek’s disease virus as Curcumin 26.1±1.7a, Bottle gourd 25.62 ±1.1a, Positive control 17.125 ±0.6b; these results indicate that both Curcumin and bottle gourd have an antiviral effect against recently isolated wild type MDV.

Keywords | Marek’s disease virus, Curcuma longa, Bottle gourd, Polymerase chain reaction SYBR green mix, Plaque reduction assay.