Advances in Animal and Veterinary Sciences

Research Article
Adv. Anim. Vet. Sci. 9(9): 1460-1465
Http://dx.doi.org/10.17582/journal.aavs/2021/9.9.1460.1465
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Mohammed Mahdi Mohammed, Tahreer Mohammed Al-Thuwaini*, Mohammed Baqur Sahib Al-Shuhaib

Department of Animal Production, College of Agriculture, Al-Qasim Green University, Al-Qasim, Babil 51001, Iraq.

Abstract | Oxidized low-density lipoprotein receptor 1 (OLR1) is the major scavenger endothelial receptor that is involved in the binding and degradation of oxidized low-density lipoproteins (Ox-LDL), hence it is responsible in controlling lipid metabolism. Genetic polymorphism in the OLR1 gene is associated with the variation in Ox-LDL of livestock. Therefore, this study aimed to investigate the association of OLR1 polymorphism with the lipid profile levels in Awassi ewes. A total of 200 sexually mature ewes, non-pregnant and non-lactation, aged between 2.5 to 5 years were included in this study. Blood samples were collected from the sheep for extracting genomic DNA, genotyping and the sequencing reaction were subsequently used to confirm the variants in amplified fragments. Blood sera were harvested to determine lipid profile assay. The result referred to the significant differences (P < 0.05) in total cholesterol, HDL-c, LDL-c levels and lipid ratio between the CC genotype compared to the CA genotype. The genotype CC correlated positively with total cholesterol (r= 0.51, P= 0.02), HDL-c (r= 0.63, P= 0.02) and negatively with LDL-c (r= -0.34, P= 0.03), TC/HDL-c (r= -0.43, P= 0.01), LDL-c/HDL-c (r= -0.38, P= 0.04), LDL-c/ TG (r= -0.58, P= 0.02), and TG/HDL-c (r= -0.64, P= 0.02). Sheep with the CC genotype had higher levels of total cholesterol and HDL-c but lower levels of LDL-c and lipid ratio thus are more favored for human consumption. In conclusion, OLR1 gene has a key functions in the regulation of lipid metabolism and affect many economic traits. Based on these outcomes, OLR1 gene represented one of the important candidate genes for fat deposition-related traits in livestock.

Keywords | Lipid profile; Ox-LDL; OLR1; polymorphism; sheep.