Advances in Animal and Veterinary Sciences

Research Article
Adv. Anim. Vet. Sci. 9(9): 1449-1455
Http://dx.doi.org/10.17582/journal.aavs/2021/9.9.1449.1455
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Al Shimaa Al Housiny Hasab El-Naby1, Mohamed Mahmoud Moustafa Kandiel1,Karima Ghonaimy Mohamed Mahmoud2*, Gamal A. El-Sisy2, Walid Tawfik Mohamed Soliman3, Yousef Fawzy Ahmed2

1Department of Theriogenology, Faculty of Veterinary Medicine, Benha University, Egypt; 2Department of Animal Reproduction and A. I., National Research Center, Dokki, Giza, Egypt; 3Veterinary Department, Egyptian Armed Forces, Nasr city, Cairo, Egypt.

Abstract | Our study aimed at addressing the question “Do spermatozoa biometric measures prove its fertility potential?”. Therefore, we carried out a comparative study of the buffalo bull’s fertility potential between Italian and Egyptian breeds based on morphometric analysis of frozen-thawed semen and in vitro fertilization. Semen was evaluated post-thawing for plasma membrane integrity, morphology, and motility. Sperm morphology was quantified using computer-assisted evaluation. The cleavage rates, morula, and blastocyst morphologies were recorded for the in vitro assessment of the fertilizing capacity. Our results show substantial differences in motility, viability, abnormalities, plasma membrane (P<0.001), and acrosome integrities (P<0.01) between the two bull breeds. Spermatozoa of Egyptian bulls are characterized by a longer head length, larger head area and a higher ellipticity. In contrast, Italian buffalo spermatozoa are characterized by a wider head base and acrosomal width, larger head perimeter, higher rugosity, and difference in head shape. The total flagellum and principal piece lengths are longer in Italian bull sperm. Midpiece (volume and width) and principal piece (volume) measures are increased in Egyptian bull sperm. In contrast, the blastocyst rate was significantly (P< 0.05) higher in Italian than Egyptian bull semen. There is no marked difference between the two breeds in cleavage and morula rates. In conclusion, the detected considerable variations in sperm biometry between the Egyptian and Italian semen/spermatozoa are attributed to the significant variations in the in vitro fertility potentials.

Keywords | Biometry, Egyptian bulls, Italian semen, Spermatozoa, IVF.