Advances in Animal and Veterinary Sciences

Research Article
Adv. Anim. Vet. Sci. 9(9): 1376-1382
Http://dx.doi.org/10.17582/journal.aavs/2021/9.9.1376.1382
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Gehan L. Abuelenain1*, Zeinab H. Fahmy1, Amal M. Elshennawy1, Azza M. Fahmy1, Eman M. Ali1, Olfat Hammam2, Abdel-Wanes A. Abdel-Aziz3

1Department of Immunology and Drug Evaluation, TBRI, Giza, Egypt; 2Department of Pathology, TBRI, Giza, Egypt; 3Department of Medicinal Chemistry, TBRI, Giza, Egypt, Affiliated with Fatima College of Health Sciences, Al Ain, United Arab Emirates.

Abstract | Trichinellosis is one of the zoonotic diseases that cause health and economic problems worldwide. Albendazole is the approved anti-parasite medicine but resisted by the larvae that reside in the muscle cells, transforming them into tumor-like nest cells. This study aimed to investigate the potential effect of two medicinal plants, Lepidium sativum (Garden cress) and Commiphora molmol (Myrrh), on trichinellosis because of their folk utilization in the Middle East and some other African countries. Male Swiss albino mice (n=106) were randomly placed into two groups postinfection with Trichinella spiralis larvae (3.0 x102). One group of infected mice received either Garden cress (500mg/kg), Myrrh (500mg/kg), or mono-combined with Albendazole (50mg/kg) on day zero for three consecutive days, and was decapitated on day seven post-infection (pi). The other group of mice received the same regimens on day 30 and were sacrificed on day 35 pi. Three control groups ran in parallel: Albendazole treated-infected mice, non-infected, and infected mice. The highest reduction of worm counts was due to Albendazole and its combinations with either Garden cress or Myrrh. However, the most significant impact on larvae was in favor of Myrrh alone or combined with Albendazole. The muscular tissues were ameliorated with Myrrh’s treatment protocols, which induced the nest cells’ larvae destruction. The biochemical tests indicated the engagement of vital proteins in the action mode of all treatments. Therefore, we concluded that Myrrh might be a valuable future antagonizing drug of trichinellosis. However, further studies on the administered concentrations of both Garden cress and Myrrh are vital to verify the optimum dose with the best action mode on the host responses.

Keywords | Trichinella spiralis, Trichinellosis, Drug discovery, Medicinal plants, Garden cress, Myrrh, Lepidium sativum, Commiphora molmol