Advances in Animal and Veterinary Sciences

Short Communication
Adv. Anim. Vet. Sci. 9(9): 1342-1346
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Rabiga S. Uakhit1,2, Ludmila A. Lider2, Ainura M. Smagulova1, Sergey V. Leontyev4, Sarsenbay K. Abdrakhmanov3, Vladimir S. Kiyan1*

1Research Platform of Agricultural Biotechnology, 2Department of Veterinary Medicine, 3Department of Veterinary Sanitation, Saken Seifullin Kazakh Agrotechnical University, Nur-Sultan, 010011, Kazakhstan; 4Facility of biology and technology, Department of biology, Bioresources and Aquaculture, Novosibirsk State Agrarian University.

Abstract | The clinical manifestations of dirofilariasis are usually different and depend on the species: D. immitis is located on the right side of the heart and pulmonary artery. On the other hand, D. repens is localized in the subcutaneous tissue. According to the literature, dirofilariasis is a filaroid nematode present in animals and humans, which is mainly transmitted by mosquitoes. This is a rare case of finding helminths in the heart cavity of a wolf and raises questions about its distribution area in Kazakhstan. To determine the genotype of Dirofilaria spp. found in the heart of a wild wolf, identification was carried out by PCR using the species-specific primer SSU rRNA, as a result of which amplicons of 875 bp were obtained. The results of the study showed that the nucleotide sequence of the studied species was identified as D. repens, and this sequence was deposited in the NCBI GenBank database (accession number MT877205.1). The research results revealed the imperfection of the differential identification of Dirofilaria spp parasites only by their morphological structure and confirm the need to use molecular identification methods.

Keywords | Dirofilaria repens, Wolf, Heart, Molecular identification, SSU gene