Advances in Animal and Veterinary Sciences

Adv. Anim. Vet. Sci. 9(9): 1302-1310
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Aagosh Verma1*, Anuradha Singh2, Suchitra Sharma3, Ruchika Atri3

1Career College, Bhopal, India; 2Associate Professor, Department of Zoology, University of Rajasthan, Jaipur, India; 3Research Scholar, Department of Zoology, University of Rajasthan, Jaipur, India.

Abstract | Feed derived pathogens are a big concern for human health as it is the beginning of the food safety chain in the farm-to-fork model. Less attention have been paid and only few studies so far have been designed to access the prevalence of these harmful bacterial colonies in animal feed. Therefore, the study was designed to assess the prevalence of ESBL-producing E. coli in cattle feed from different cattle feed mills situated in Bhopal and nearby. The objectives addressed were (1) to isolate and molecularly identify E. coli from cattle feed samples of different feed mills in Bhopal and nearby areas (2) to evaluate the antimicrobial susceptibility patterns of recovered E. coli (3) identification of genes bla CTXM grp 1,3,9, bla SHV and bla TEM in Extended-spectrum lactamase (ESBL)-producing Escherichia coli strains using PCR. A total of 200 feed samples collected which yielded 38 (19%) E.coli contaminated feed lots. Ninteen samples of feed pellets and 19 of mash feed samples were found to carrier of ESBL positive E. coli through API20E and PCR confirmation. CTXM group 2 is present in all 38 strains. E. coli isolates showed highest resistant towards drug Tetracycline and Chloramphenicol of 78.9%. On other side, they were found sensitive with Ampicillin (92.1%), Imipenem (78.9%) and third generation cephalosporins. Hygiene conditions in Feed mill industry, feed farms, trough, storage conditions, humidity, temperature, conditions in pellet machines all plays important role in E.coli contaminations. More such studies, survey and deep microbiological analysis should be promoted and is required in this field to enhance proper functioning of dairy food industries.

Keywords | Animal feed, ESBL producing E.coli, Antimicrobial susceptibility, API20E