Advances in Animal and Veterinary Sciences

Research Article
Adv. Anim. Vet. Sci. 8(3): 229-233
Http://dx.doi.org/10.17582/journal.aavs/2020/8.3.229.233
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Son Trinh Hong2, Vinh Nguyen Thi1, Pham Pham Duy2, Luc Do Duc1, Dang Pham Kim1, Giang Nguyen Thi Phuong1, Tuan Nguyen Ngoc Minh3, Thinh Nguyen Hoang1*

1Faculty of Animal Science, Vietnam National University of Agriculture, Vietnam; 2Thuy Phuong Pig Research and Development Center, Vietnam National Institute of Animal Science, Vietnam; 3Hung Vuong University, Vietnam.

Abstract | Prolactin receptor (PRLR) and vertnin (VRTN) gene is considered as genetic marker for teat, vertebrae and ribs number in pigs. In this study, the polymorphisms of these genes was investigated in Landrace, Yorkshire and Meishan purebred pigs. A total of 102 animals including 37 Landrace, 35 Yorkshire and 30 Meishan were collected from Thuy Phuong Pig Research and Development Center, Vietnam National Institute of Animal Science, Vietnam. Pig individuals were genotyped with PCR and PCR-RFLP methods. Allelic and genotypic frequencies were calculated. Hardy-Weinberg equilibrium (HWE) was performed using χ2-test. For PRLR, 3 genotypes (AA, AB and BB) were detected and the most abundant genotype was the heterozygous one. The frequencies of expected genotype AA were 0.35, 0.40, and 0.17 for Landrace, Yorkshire, and Meishan, respectively. Frequencies of allele A were 0.61 (Landrace), 0.69 (Yorkshire), and 0.59 (Meishan). The loci were in HWE for all group of pigs except Meishan. For VRTN, those values for QQ genotype were 0.30 (Landrace), 0.37 (Yorkshire), and 0.10 (Meishan), while Q allele frequencies were 0.43 (Landrace), 0.57 (Yorkshire), and 0.22 (Meishan). HWE was detected for Meishan Pig.


Keywords | Prolactin receptor, Vertnin gene, Teat, Vertebrae, Rib