Hmga2 regulates stem cell proliferation and pluripotency
Hmga2 controls the regulation of cell growth inhibitors (P16, P19) and accordingly maintains or accelerates the proliferation of stem cells. On the other side hmga2 plays a crucial role in up regulation of pluripotency specific genes hence retains the pluripotency features of stem cells.

Relative pluripotency genes expression by stimulated and unstimulated canine adipose mesenchymal stem cells (cASCs) with hmga2 protein (50, 100 ng/ml) after real time PCR
OCT4, KLF4, cMYC, HMGA2 and SOX2 are expressed by cASCs but no difference was detected at the used concentrations.

Proliferation assay of stimulated cASCs with hmga proteins
Hmga1 has significant inhibiting effect on cell proliferation when used alone (10–200 ng/ml) or combined with hmga2 (100, 200 ng/ml). Contrarily, there is no significant differences in cell proliferation are observed in hmga2-treated groups compared to un-stimulated cells. One-way analysis of variance and Tukey test was performed for statistical comparison (significance levels: *P<0.05; ** P<0.01; *** P<0.001)