SPF chicken groups vaccination and challenge details. Chicks were vaccinated and challenged 28 days after vaccination according to their vaccination. Groups 4, 5, and 6 were divided at the time of challenge into 2 groups (n=10 each), kept in separate isolators and challenged with either NDV or IBV. Swabs were collected 3, 5, and 7DPC for viral shedding determination. For IBV vaccinated groups ciliostasis test was performed 7DPC 

Serological monitoring and ciliostasis for vaccinated and challenged chicken groups. A) NDV HI titers in vaccinated groups. Response was fast, started one-week post vaccination (wpv) and reached its paek 4wpv. B) IBV ELISA titers in IBV-vaccinated groups. Group IV antibodies were significantly higher than group V. The highest ELISA titer was recorded 6wpv in group IV sera. C) IBV HI titers, peak titers have been recorded 5wpv. Vaccinated groups differed significantly from challenged non-vaccinated group (group VI) but not from each other. D) IBV protection percentages based on ciliary protection; vaccinated groups scored ciliostasis scores around 11 where scores ≤ 20 are considered protective. Challenged non-vaccinated group ciliostasis score was 37 indicating lack of protection. Protection percentages in vaccinated groups was around 70.